Bodily injury and/or harm to property can occur if proper operating procedures are not understood and followed.
Disconnect unit from power supply to prevent electrical shock while handling or moving unit.
Do not touch conducting buffer solution (buffer) or electrical leads until unit is disconnected from power supply.
If unit or electrical leads are damaged or cracked, discontinue use immediately.
Only clean unit with mild detergent and water.
Never apply organic solvents or ethanol to clean unit as doing so will crack/damage the unit.
Never microwave, bake or autoclave unit as temperatures above 70°C will crack/damage the unit.
1. Understand, read and perform the steps outlined in this operating manual to insure proper safe operating techniques.
2. Qualified and trained personnel required to operating unit for this symbol: Electrical Hazard.
3. Unplug unit from power supply when performing service or maintenance.
4. Surfaces of unit may become hot, use caution while handling unit to prevent burns to personnel or environment.
5. PPE (personal protective equipment – i.e. googles, gloves, clothing, etc.) must be worn while using unit.
6. Practice proper hygiene techniques at all times.
7. Practice safety for one’s self at all times.
8. Do not exceed the ‘MAX FILL LINE’ with buffer. Electrical connection holes are NOT sealed and will leak liquid if overfilled.
9. Do not touch electrical leads or conducting buffer solution on unit.
Review the parts list below before proceduing.
1x Gel Electrophoresis (GE) Box
1x Gel Tray
1x Gel Cover
1x Power Supply Cable
1x Gel Comb (10 tooth - 1.5mm)
Insert gel tray into GE box as shown below.
The gasketed ends of the gel tray will be pressed against and apply pressure to the walls of the GE box.
First, place one side in first then using pressure push opposite side in.
The walls of the GE box will flex – this is normal and will return back the original configuration when the gel tray is removed.
IMPORTANT: center and level the gel tray before proceeding.
Use any type of tape that does not leave a residue upon removing (transparent tape, masking tape, etc.) to create two temporary walls on the gel tray. Apply a length of tape long enough to cover the length of one side of the gel tray with enough overhang to extend past the edge to safely secure the tape to the tray. Apply the tape as shown below. The adhesive portion of the tape will face towards the tray. Perform this task on the opposite side of the gel tray. Secured tape will form a temporary leak-proof wall. Test with water for any leaks first.
In this step, only use electrophoresis-grade buffer and electrophoresis-grade agarose. The buffer and agarose will be mixed dependent using the experiment’s requirements using a heat source (microwave, etc.) until mixture is homogeneous. Do not pour bubbling mixture into gel tray/gel box. Mixture must be below 60°C before casting into gel tray/gel box.
Pour ~60mL of agarose mixture (from Step 2) into the gel tray that was prepared from (Step 1a or Step 1b). Insert the gel comb onto the gel tray (in the designated gel comb slot of the gel tray) right after pouring the mixture.
Wait ~20-30 minutes or until gel mixture has hardened and remove the gel comb from the gel tray. Remove the gel tray from the gel box (if using Step 1a) or remove tape from the gel tray (if using Step 1b). Rotate gel tray 90° so that the walls of the gel tray are parallel with the long side of the gel box (gasketed ends of the gel tray are facing towards the short side of the gel box).
Hand pour electrophoresis-grade running buffer into gel box up to “MAX FILL LINE”. Do not exceed the “MAX FILL LINE” as the electrical connection ports are not sealed and will leak buffer.
Prepare samples using experiment’s protocols. Pipet sample into cavities or wells in the gel. Apply colored dye to sample for visual monitoring during gel electrophoresis experiment run.
Attach power cables to electrical leads on the gel box. The negative (black) power cable will be placed closest to the end of the gel tray with the ‘START HERE’ sticker. The positive (red) power cable will be placed furthest away from the end of the gel tray with the ‘START HERE’ sticker. Slip gel cover into the gel box’s top grooves. Insert power cables into an electrophoresis power supply and turn on power supply. Be sure to periodically monitor the gel’s progress by stopping the power supply and removing the gel cover.
Turn off power supply when the dye reaches near the end of the gel or specified in the experiment’s protocols. Remove power cables from power supply and gel box. Remove gel cover from the gel box. Grab onto the ends of the overhangs of the gaskets and pull the gel tray out of the gel box. The gel tray may be difficult to remove from the gel box due to the tight seal therefore pull on the gaskets in an alternating fashion will lift the tray easier. Gel box, gel tray, gel comb and gel cover must be rinsed with detergent and water after use.
Need a replacement? We’ve got you covered! All the parts are replaceable! See more by contacting the distributor.
• Replacement ALT SmartCast B1A Gel Box
Krackeler Catalog #: ALTWTNUS00002
• Replacement ALT SmartCast B1A Gel Tray
Krackeler Catalog #: ALTWTNUS00003
• Replacement ALT SmartCast B1A Gel Cover
Krackeler Catalog #: ALTWTNUS00004
• Replacement Gasket for ALT SmartCast B1A
Krackeler Catalog #: ALTWTNUS00005
• Replacement insulated wire and electrical connectors for ALT SmartCast B1A
Krackeler Catalog #: ALTWTNUS00006
• ALT SmartCast B1A Gel Comb - 10 tooth 1.5mm
Krackeler Catalog #: ALTWTNUS00007